Pdf the development of near isogenic lines nils through repeated backcrossing of genetically distinct parental lines is rather. Starch granule size distribution in wheat endosperm. Tortillas were prepared from each deletion line and the parent lines. Cucumis melo, introgression line, qtl, g e interaction, heritability abstract. Pdf qtl mapping with nearisogenic lines in maize researchgate. Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never. Wl711 developed at the school of biotechnology were used along with the donor and the recurrent parents for identifying rapd markers linked to the two genes.
Nearisogenic lines nils integrated breeding platform. The development and application of nearisogenic lines for. Nearisogenic lines nils with single blast resistance genes were developed by backcrossing four donor cultivars to the recurrent parent. Application of a library of near isogenic lines to understand context dependent expression of qtl for grain.
Registration of four nearisogenic soybean lines of g00. Use of nearisogenic wheat lines to determine the glutenin. The nearisogenic lines nils inbred lines are usually developed for qtl mapping purpose. In arabidopsis recombinant inbred line ril populations are widely used for quantitative trait locus qtl analyses.
Application of a library of near isogenic lines to understand context. The amylose content, size distribution, and processing properties of isolated starch granules were compared between eight near. Production of nearisogenic lines and marked monosomic lines in. Identification of molecular markers linked to leaf rust resistance genes in wheat and their detection in the local nearisogenic line. An additional file shows the details of experimental conditions in more detail see additional file 1. Comparison of near isogenic lines of wheat showed that the wheat resistance genes is localized within a segment of the short arm of chromosome 2n from aegilops ventricosa zhuk. Development of nearisogenic lines in a parthenogenetically. Use of nearisogenic lines derived by backcrossing or selfing to map. Sixteen nearisogenic lines nils carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of. The progeny with the traitofinterest are then selected for backcrossing to the reference line.
Multiple nearisogenic lines targeting a qtl hotspot of. Estimatingthegeneticarchitectureoffruitquality traits in. The control line was derived from the same cross and backcrossing procedures as described above, but was homozygous for the b73 allele at each of the 19 rflp loci. Chennav translocated into common wheat triticum aestivum l. Characterization and identification of cold tolerant near.
Development of a nearisogenic line population of arabidopsis. Pdf nearisogenic lines nils constitute valuable tools in genetic investigations and plant breeding programs. The relationships between development and low temperature. Isogenic definition is characterized by essentially identical genes. The effects of bt and near isogenic lines of rice were studied in two laboratory experiments. Near isogenic lines nils are derived via repeated backcrossing of genetically distinct parental lines, most commonly with the aim of transferring a single chromosomal region of the donor parent. Inheritance of blast resistance in nearisogenic lines of rice.
An aegilops ventricosa translocation confers resistance. Each of the 89 nils, the control line, tx303, and b73 was test crossed to the inbred line mo17 for evaluation. Abstractto exploit the genetic mechanism of cold tolerance in rice, cold tolerant nearisogenic lines nils. Pdf a set of 89 nearisogenic lines nils of maize was created using marker assisted selection. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference. This is a new line that differs from its parent in only one genomic location. The elimination of certain hmwgs alleles alter distinct but critical aspects of tortilla quality such as diameter, shelf stability, and overall quality. We characterized a set of isogenic genetic stocks representing single and pairwise substitutions of spring alleles at the. Backcross populations and near isogenic lines article pdf available in methods in molecular biology clifton, n. Using the marker identified for that qtl, backcrosses are made to the recurrent parent usually the cultivated variety.
Nearisogenic wheat lines in which one or more of these loci were absent or deleted were used. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference line is initiated. Growth, reproduction, and superoxide dismutase activity were unaffected both in the petridish and soil microcosm experiment. Nearisogenic lines nils are a valuable resource for detecting linkages between qualitative trait loci and molecular markers. Nineteen genomic regions, identified by restriction.
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